| Agency | | City |
| Bay Town Trolly | | Panama City |
| Broward County Transit | | Plantation |
| Charlotte County Transit Division | | Punta Gorda |
| Citrus County Transit | | Lecanto |
| Collier Area Transit (CAT) | | Naples |
| Escambia County Area Transit | | Pensacola |
| Flagler County Public Transportation | | Bunnell |
| Gainesville Regional Transit System (RTS) | | Gainesville |
| Hernando (Trans-Hernando Express) | | Brooksville |
| Hillsborough Area Regional Transit Authority (HART) | | Tampa |
| Indian River (GoLine) | | Vero Beach |
| Jacksonville Transportation Authority (JTA) | | Jacksonville |
| Lake County Public Transportation (LakeXpress) | | Tavares |
| Citrus Connection | | Lakeland |
| LeeTran | | Fort Myers |
| LYNX Transit | | Orlando |
| Manatee County Area Transit (MCAT) | | Sarasota, Bradenton |
| Martin County (MARTY) | | Stuart |
| Miami-Dade Transit | | Miami |
| Okaloosa County (EC Rider) | | Fort Walton Beach |
| Palm Trans | | West Palm Beach |
| Pasco County Public Transportation | | Port Richey |
| Pinellas Suncoast Transit Authority | | St. Petersburg |
| St. Johns COA (Sunshine Bus) | | St. Augustine |
| St. Lucie COA (Treasure Coast Connector) | | Fort Pierce |
| Sarasota County Area Transit (SCAT) | | Sarasota |
| South Florida Regional Transportation Authority (Tri-Rail) | | Pompano Beach |
| Space Coast Area Transit | | Cocoa |
| Star Metro | | Tallahassee |
| SunRail (Central Florida Commuter Rail) | | Sanford |
| SunTran | | Ocala |
| VOTRAN | | South Daytona |
| City of Fort Lauderdale | | Fort Lauderdale |
| vRide, Inc. | | Miami Lakes |
| Looper Trolley | | St. Petersburg |
| Tampa Bay Area Regional Transportation Authority (TBARTA) | | Tampa |
| GoLine (Indian River County Transit) | | Vero Beach |
| | | |
